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In summary, stepwise oxidation of one molecule of acetyl CoA through the citric acid cycle releases 2 molecules of C0 2 . The free energy released during the oxidation reactions is conserved in the form of 3 molecules of XADII and 1 molecule of FADIi 2 . One molecule of ATP is also produced by the substrate-level phosphorylation. Two molecules of water are consumed during the operation of the cycle. Although formation of acetyl CoA from pyruvate is not part of the citric acid cycle, one should be reminded here that during this reaction 1 molecule of C0 2 is released and one molecule of XADH is produced. Therefore, oxidation of one molecule of pyruvate inside the mitochondrion produces, in total, 4 molecules of NADII, 1 molecule of FADH 2 and 1 molecule of ATP, and 3 molecules of C0 2 are also released.